Travel time from Osaka is approximately 2 hours 30 minutes. In the city's downtown shopping district, Tottori Station offers regularly scheduled local and express train service on the JR West rail line. These two universities are not to be confused with the 2-year junior college in the prefecture, Tottori College, which is located in the central city of Kurayoshi. The privately funded Tottori University of Environmental Studies is located in the south-eastern part of Tottori city, near the town of Yazu. The main campus of Tottori University, a national public university, is located next to Koyama Lake on the west end of the city. Ketaka District was dissolved as a result of this merger. On November 1, 2004, the town of Kokufu, the village of Fukube (both from Iwami District), the towns of Aoya, Ketaka and Shikano (all from Ketaka District), the towns of Kawahara and Mochigase and the village of Saji (all from Yazu District) were merged into Tottori. Redistricting (" gappei") of the city's borders in November 2004 increased its size to include a number of surrounding areas. Exchange students who come to Tottori can experience countryside life and enjoy traditional Japanese customs through the many events organized by the Tottori AFS. The organization AFS ( AFS Intercultural Programs) for exchange students is relatively developed in Tottori. Most of the downtown area was destroyed by the Tottori earthquake of September 10, 1943, which killed over 1000 people. Tottori was incorporated as a city on October 1, 1889. The event lasts an entire weekend and some top names on the national DJ circuit are invited to perform. An exceptionally big example of a Shan-shan umbrella graces the main foyer of Tottori Station.Īt the beginning of every summer, Tottori is host to one of the biggest beach parties in the country, the San In Beach Party. The city also hosts the prefecturally famous Shan-shan festival in the summer, which features teams of people dressing up and dancing with large umbrellas the name 'Shan-shan' is said to come from the sound made by the small bells and pieces of metal attached to the umbrellas, which are very large. In the vicinity are temples, museums, and public parks. It is open to the public, and is the site of the Castle Festival in autumn each year. Its centre is the now ruined Tottori Castle, once the property of the Ikeda clan daimyō who ruled the Tottori Domain during the Edo period. Around this mountain lies the oldest part of the city. The city's main street (Wakasa, or "young cherry blossom" street) runs north from the station and terminates at the foot of the Kyushouzan ("eternal pine") mountain. These findings indicate that Pt-blue is a useful, safe, and easily obtainable electron stain that is an alternative to UA for TEM preparations.Climate data for Tottori (1991−2020 normals, extremes 1943−present)Ĭlimate data for Aoya, Tottori (1991−2020 normals, extremes 1978−present) coli, allowing the visualization of a flagellum. The present study also revealed that Pt-blue could be used for the negative staining of E. When post-embedding immunoelectron microscopy was performed in ultrathin sections of HeLa cells embedded in Lowicryl K4M, the localization of Ki-67 protein was sufficiently detected even after Pt-blue and Pb staining. In longitudinal and transverse sections of budding influenza A viruses, a specific arrangement of rod-like structures, which correspond to the ribonucleoprotein complexes, was clearly shown in each virion stained with Pt-blue and Pb. Glycogen granules in the hepatic parenchymal cells were particularly electron dense in Pt-blue stained sections compared with those treated with UA. Almost all cell organelles were clearly observed with high contrast in these sections. In ultrathin sections of the rat liver and renal glomerulus, Pt-blue provided good contrast images, especially in double staining combined with a lead stain (Pb). The effect of Pt-blue as an electron stain was then examined not only for positive staining of conventional ultrathin resin sections and counterstaining of post-embedding immuno-electron microscopy but also for negative staining. When Pt-blue was dried on a microgrid and observed by TEM it showed a uniform appearance with tiny particles less than 1 nm in diameter. Pt-blue was prepared from a reaction of cis-dichlorodiamine-platinum (II) (cis-platin) with thymidine. This paper introduces an aqueous solution of platinum blue (Pt-blue) as an alternative to uranyl acetate (UA) for staining in transmission electron microscopy (TEM).
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